DNA replication origins, ORC/DNA interaction, and assembly of pre-replication complex in eukaryotes

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DNA replication origins, ORC/DNA interaction, and assembly of pre-replication complex in eukaryotes.

Chromosomal DNA replication in eukaryotic cells is highly complicated and sophisticatedly regulated. Owing to its large size, a typical eukaryotic genome contains hundreds to tens of thousands of initiation sites called DNA replication origins where DNA synthesis takes place. Multiple initiation sites remove the constraint of a genome size because only a certain amount of DNA can be replicated ...

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Origins of DNA replication (ORIs) occur at defined regions in the genome. Although DNA sequence defines the position of ORIs in budding yeast, the factors for ORI specification remain elusive in metazoa. Several methods have been used recently to map ORIs in metazoan genomes with the hope that features for ORI specification might emerge. These methods are reviewed here with analysis of their ad...

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ژورنال

عنوان ژورنال: Acta Biochimica et Biophysica Sinica

سال: 2010

ISSN: 1672-9145,1745-7270

DOI: 10.1093/abbs/gmq048